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Appl Environ Microbiol. 1967 March; 15(2): 292-295
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Isolation and Purification of Staphylococcal Lipase¹

Department of Food Science, Michigan State University, East Lansing, Michigan

ABSTRACT

An extracellular lipase was isolated from the cell-free supernatant fluid of a 24-hr culture of Staphylococcus aureus grown in Trypticase Soy Broth at 37 C with continuous agitation. The purification was achieved by precipitation with alcohol followed by differential precipitation at pH 8.6 and 4.3. Subsequent purification with Sephadex G 200 and BioGel 300 yielded a preparation which showed a 350- to 450-fold increase in specific activity over the original cell-free supernatant fluid. The purified lipase was homogeneous over a BioGel 300 column and showed a single peak on electrophoresis in a Veronal buffer (pH 8.6, /2 = 0.1). The electrophoretic mobility was -7.78 x 10^-5 cm² per v per sec.

² Present address: Department of Poultry Husbandry, Cornell University, Ithaca, N.Y.

¹ Published with the approval of the Director of the Michigan Agricultural Experiment Station as Journal Article 3920.