This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sasaki, Y. Articles by Takao, S. Search for Related Content PubMed Articles by Sasaki, Y. Articles by Takao, S. Agricola Articles by Sasaki, Y. Articles by Takao, S.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1967 March; 15(2): 373-377
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Organic Acid Production by Basidiomycetes

III. Cultural Conditions for L-Malic Acid Production

Laboratory of Applied Microbiology, Faculty of Agriculture, Hokkaido University, Sapporo, Japan

ABSTRACT

Cultural conditions were examined for the purpose of increasing yields of L-malic acid by the Basidiomycetes Schizophyllum commune and Merulius tremellosus, which have the ability to produce this acid as a main product in CaCO₃-containing medium in shaken culture. The most favorable nitrogen sources selected were 0.3% (NH₄)₂SO₄ and 0.18% NH₄Cl. Effective combinations of inorganic salts in the medium were 0.1% KH₂PO₄, 0.05% MgSO₄·7H₂O, and 0.05% KCl, and suitable concentrations of glucose were 5 to 10%. Several nonionic surface-active agents promoted the filamentous mycelial growth of these strains and increased acid production. In particular, Tween 80 in 0.3% concentration markedly stimulated malic acid production by S. commune, and yields greater than 50% based on available glucose, were obtained after 10 to 14 days. Acid production by M. tremellosus was stimulated most with 0.5% Carbowax 4000 (polyethylene glycol), and the resultant yields were more than 40%.