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Appl Environ Microbiol. 1967 May; 15(3): 551-555
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Improved Assay Method for Phospholipase C

Susumu Kurioka and Pinghui V. Liu

Department of Microbiology, University of Louisville, School of Medicine, Louisville, Kentucky 40202

ABSTRACT

A lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase C in the presence of calcium ion more rapidly than were any other lecithin sols. The inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase C reaction mixture. A simple and accurate assay method for phospholipase C was developed with the sol and the alkaline phosphatase.

Appl Environ Microbiol. 1967 May; 15(3): 551-555
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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Copyright © 1967 by the American Society for Microbiology. All rights reserved.