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Appl Environ Microbiol. 1967 May; 15(3): 569-574
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Dechlorination of 1,1,1-Trichloro-2,2-bis(p-chlorophenyl)ethane by Aerobacter aerogenes

I. Metabolic Products

U.S. Fish and Wildlife Service, Fish-Pesticide Research Laboratory, Columbia, Missouri

ABSTRACT

Whole cells or cell-free extracts of Aerobacter aerogenes catalyze the degradation of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) in vitro to at least seven metabolites: 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE); 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (DDD); 1-chloro-2,2-bis(p-chlorophenyl)ethylene (DDMU); 1-chloro-2,2-bis(p-chlorophenyl)ethane (DDMS); unsym-bis(p-chlorophenyl)ethylene (DDNU); 2,2-bis(p-chlorophenyl)acetate (DDA); and 4,4'-dichlorobenzophenone (DBP). The use of metabolic inhibitors together with pH and temperature studies indicated that discrete enzymes are involved. By use of the technique of sequential analysis, the metabolic pathway was shown to be: DDT DDD DDMU DDMS DDNU DDA DBP, or DDT DDE. Dechlorination was marginally enhanced by light-activated flavin mononucleotide.

¹ Present address: U.S. Fish and Wildlife Service, Western Fish Disease Laboratory, Sand Point Naval Air Station, Seattle, Wash. 98115.

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