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Appl Environ Microbiol. 1967 May; 15(3): 594-596
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Effect of Imput Multiplicity and Tissue Cell Concentration on Growth of Rift Valley Fever Virus

Department of the Army, Fort Detrick, Frederick, Maryland 21701

ABSTRACT

The effects of virus input multiplicity and of tissue cell concentration upon the growth of Rift Valley fever virus in L cells (Earle) were determined. The titers obtained in suspension cultures with cells obtained from two separate laboratories were significantly different. With both monolayer culture and suspension culture systems, a virus input multiplicity of 2.5 resulted in the greatest proliferation of virus. Optimal viral yields were obtained in suspension cultures containing 4 x 10⁵ tissue cells per ml of suspension.