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Appl Environ Microbiol. 1967 July; 15(4): 723-725
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Application of Paper Chromatograms to the Study of Inducers of Bacteriophage in Escherichia coli

Research Division, Bristol Laboratories (Division of Bristol-Myers Company), Syracuse, New York 13201

ABSTRACT

A procedure has been developed whereby paper chromatograms of agents which induce bacteriophage in Escherichia coli can be developed using bioautographs with a lysogenic test system. Well-defined plaque-forming zones are produced indicating the area on the paper chromatogram where the active inducing material can be located. A mixture of the bacteriophage-inducing antibiotic, mitomycin C, and the noninducing antibiotic, paromomycin, was resolved into its components on paper strips with an ethyl acetate-methanol solvent system. The location of both antibiotics could thus be readily observed. Antibacterial and inducing activities were found to be identical with a crude fermentation solid, NSC-B-158,791. The use of this procedure for resolution of multicomponent inducing activities in antibiotic beers and for characterization of active components which may be potential antitumor antibiotics is indicated.