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Appl Environ Microbiol. 1967 July; 15(4): 866-870
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Nutritional Requirements of Staphylococcus aureus S-6¹

Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina

ABSTRACT

A synthetic medium was devised for growth of Staphylococcus aureus strain S-6. The growth yield in synthetic medium was compared to that in complex medium containing an equivalent amount of protein hydrolysate. Enterotoxin B formation in the two media was also compared. The defined medium was composed of inorganic salts, 11 amino acids (glycine, valine, leucine, threonine, phenylalanine, tyrosine, cysteine, methionine, proline, arginine, and histidine), and three vitamins (thiamine, nicotinic acid, and biotin). Biotin was a growth factor requirement of S-6 when glutamic acid but not glucose was used as a carbon source. The quantity of enterotoxin B produced in the defined medium was about one-seventh of that produced in complex medium, even though the growth yields were similar.

¹ Department publication no. 155.