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Appl Environ Microbiol. 1967 September; 15(5): 975-979
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Sporulation of Bacillus stearothermophilus

Department of Microbiology, Northwestern State College, Natchitoches, Louisiana 71457
Gerber Research Center, Gerber Products Company, Fremont, Michigan 49412

ABSTRACT

A broth medium containing tryptone and manganese sulfate supported heavy sporulation of Bacillus stearothermophilus ATCC 7953 (NCA 1518) and four isolates identified as B. stearothermophilus. Maximal spore yields were obtained by use of inocula grown anaerobically in a medium containing glucose with aeration of sporulation medium via bubbling. After an extended stationary period, sporulation occurred concurrently with vegetative growth between 6 and 8 hr of incubation at 60 C. Omission of glucose from the inoculum or use of a "young" (2 hr) inoculum abolished the stationary period, but decreased spore yields. A requirement of oxygen for rapid vegetative growth and sporulation was demonstrated. Manganese (15 to 30 ppm) stimulated sporulation but did not enhance cell growth.

¹ Present address: United Foods Co., Inc., Brownsville, Tex. 78520.