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Appl Environ Microbiol. 1968 November; 16(11): 1640-1645
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Microtiter Indirect Hemagglutination Procedure for Identification of Streptococcal M-Protein Antibodies

Streptocoecal Disease Section, National Communicable Disease Center, Public Health Service, Fort Collins, Colorado 80521

ABSTRACT

A number of investigators have attempted to utilize the hemagglutination system for detection of streptococcal type-specific antibody in human sera. Cross-reactions have made the procedure unreliable without cumbersome and time-consuming manipulation of the test sera. A method is described in which a microtiter indirect hemagglutination technique, using sensitized sheep erythrocytes, is sensitive, specific, and reliable for titration of type-specific antibody after naturally acquired or induced streptococcal infection.