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Appl Environ Microbiol. 1968 November; 16(11): 1655-1662
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Modified Biochemical Tests for Characterization of L-Phase Variants of Bacteria¹

Department of Microbiology, University of Maryland, College Park, Maryland 20740
Department of Bacteriology, Walter Reed Army Institute of Research, Washington, D. C. 20012

ABSTRACT

Biochemical capabilities of bacterial and L-phase organisms of 15 bacterial strains were examined by using a variety of modified routine diagnostic biochemical tests. The results demonstrated that 13 of the tests examined were suitable for use with L-phase variants, that L-phase variants and revertant bacterial phase organisms retained diagnostically significant capabilities of the respective bacterial or L-phase organisms from which they were derived, and that the 13 biochemical tests could be usefully employed to relate a given L-phase variant to a given bacterial phase organism, to distinguish L-phase variants of different species, and to aid in the identification of nonreverting L-phase variants.

¹ Submitted by R. L. Cohen to the University of Maryland, College Park, Md., in partial fulfillment of the requirements for the Ph.D. degree.