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Appl Environ Microbiol. 1968 November; 16(11): 1695-1698
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Detection of Salmonella by a Single-Culture Technique

Department of Epidemiology and Preventive Medicine, University of California, Davis, California 95616

ABSTRACT

Dulcitol-selenite enrichment medium in a motility flask was used for the detection of Salmonella in food. A drop in pH of the dulcitol-selenite enrichment motility broth indicated the presence of Salmonella; this phenomenon was confirmed by fluorescent-antibody staining. A complete correlation was found between fluorescent-antibody staining and recovery on Brilliant Green agar. Testing of 332 samples of 8 different kinds of foods and feeds indicated no significant difference in sensitivity between the new technique and a conventional Salmonella detection technique. The new technique permitted detection of even small numbers of Salmonella in 1 to 2 days.

¹ Present address: Food Products Division, Union Carbide Corporation, Chicago, Ill.