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Appl Environ Microbiol. 1968 November; 16(11): 1779-1781
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Freeze-drying Various Strains of Shigella

Walter Reed Army Institute of Research, Washington, D.C. 20012

ABSTRACT

Of six candidate strains of Shigella prepared in Brain Heart Infusion broth as freeze-dried vaccine, low survival rates were obtained with two of the most promising strains. Survival rates with these two strains were increased to acceptable levels when the organisms were suspended in a medium consisting of 8.2% sucrose, 0.01 M phosphate, 0.07% monosodium glutamate, and 2.5% human serum albumin. Alteration of the freezing temperature did not improve the recovery rates significantly.