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Appl Environ Microbiol. 1968 December; 16(12): 1822-1825
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of Streptococcal M-Typing Antisera

I. Antigenic Response in Different Breeds of Rabbits

Streptococcus Unit, Laboratory Program, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

M-antigens of seven M-types of group A streptococci were used to compare antigenic response in six breeds of rabbits: New Zealand White, New Zealand Red, Dutch, Googy, California, and Palomino. No appreciable differences among breeds were demonstrated. M-types used for immunization varied considerably in their ability to evoke a response of an antibody which met rigid criteria for streptococcal M-typing antisera. The importance of the injection and bleeding schedule and absorption conditions for preparing type-specific absorbed antisera were demonstrated. Evaluating and processing sera from individual animals at an optimal time rather than using pooled sera enhanced the possibilities for preparing satisfactory type-specific antisera.