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Appl Environ Microbiol. 1968 December; 16(12): 1847-1852
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of D-Mannitol and Glycerol by Yeasts

Noda Institute for Scientific Research, Noda-shi, Chiba-ken, Japan

ABSTRACT

D-Mannitol has not so far been known as a major product of sugar metabolism by yeasts. Three yeast strains, a newly isolated yeast from soy-sauce mash, Torulopsis versatilis, and T. anomala, were found to be good mannitol producers. Under optimal conditions, the isolate produced mannitol at good yield of 30% of the sugar consumed. Glucose, fructose, mannose, galactose, maltose, glycerol, and xylitol were suitable substrates for mannitol formation. High concentrations of yeast extract, Casamino Acids, NaCl, and KCl in media affected significantly the mannitol yield, whereas high levels of inorganic phosphate did not show any detrimental effect.