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Appl Environ Microbiol. 1968 February; 16(2): 232-235
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Improvement of a Continuous-Culture Apparatus for Long-Term Use¹

Department of Food Science, College of Fisheries, University of Washington, Seattle, Washington 98105

ABSTRACT

A glass and plastic apparatus was designed to meet requirements for continuous culture of cells. Some of the improvements incorporated into this apparatus include an all-glass growth vessel with a self-cleaning bottom, special compression fittings to connect tubing to glass tubing, a removable yield reservoir, and a nonwetting gas exhaust assembly. All portions of the system can be autoclaved for sterilization, and medium bottles and pump lines are replaced aseptically.

² Present address: State of Hawaii, Department of Agriculture, Honolulu, Hawaii 96814.

¹ Contribution no. 277 from the College of Fisheries, University of Washington, Seattle.