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Appl Environ Microbiol. 1968 February; 16(2): 271-275
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Fluorescent-Antibody Reagent for the Identification of Cryptococcus neoformans¹

National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

A sensitive and diagnostically applicable conjugate for the rapid and accurate detection and identification of Cryptococcus neoformans has been developed. C. neoformans rabbit antisera were produced by 14 daily intravenous injections of 36 million cells for a total dosage of approximately 500 million cells. Cross-staining reactions with species of Cryptococcus other than C. neoformans, as well as with Candida species, were eliminated by adsorption of the C. neoformans conjugate with cells of C. diffluens and C. krusei.

² Present address: Pennsylvania State Department of Public Health, Philadelphia, Pa. 19130.

¹ A portion of a dissertation submitted by the senior author to the University of North Carolina in partial fulfillment of the requirements for the degree of Doctor of Public Health in the Department of Parasitology in the School of Public Health.