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Appl Environ Microbiol. 1968 February; 16(2): 330-334
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Plate-Dilution Frequency Technique for Assay of Microbial Ecology1

R. F. Harris and L. E. Sommers

Department of Soils, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The plate-dilution frequency technique described facilitates simultaneous enumeration of a wide range of physiologically different microorganisms in complex systems with a precision comparable to dilution tube (most probable number) methods. Replicate microsamples are inoculated from each member of a dilution series onto areas delineated on plates of prepoured solid media; the plates are incubated, and the occurrence of growth or specific biochemical transformation is recorded for each inoculated area. Microbial enumeration is accomplished by reference to appropriate tables. Details of the experimental procedures are described, and tables are presented from which microbial numbers with 95% confidence limits can be obtained and compared for significant difference directly for 10-fold and 4-fold dilution series. Results of experiments in which microbial populations were estimated simultaneously by the plate-dilution frequency and conventional plate count methods are compared. The potential of the technique for broad-spectrum microbial assay is also discussed.

FOOTNOTES

1 Published with the approval of the Director, Wisconsin Agricultural Experiment Station, Madison.

Appl Environ Microbiol. 1968 February; 16(2): 330-334
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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Copyright © 1968 by the American Society for Microbiology. All rights reserved.