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Appl Environ Microbiol. 1968 April; 16(4): 624-627
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Differential Chromatographic Bioassay for the Gentamicin Complex

Gerald H. Wagman, Edwin M. Oden and Marvin J. Weinstein

Microbiology Department, Schering Corporation, Bloomfield, New Jersey 07003

ABSTRACT

A differential paper chromatographic assay for the components of the gentamicin complex, designated as C1, C1a, and C2, is described. The gentamicins are separated on paper strips with the lower phase of a solvent mixture consisting of chloroform, methanol, and 17% ammonium hydroxide (2: 1: 1, v/v). After chromatographic separation, the paper strips are plated against Staphylococcus aureus ATCC 6538P, and the zones of activity are quantified against known standards.

Appl Environ Microbiol. 1968 April; 16(4): 624-627
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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Copyright © 1968 by the American Society for Microbiology. All rights reserved.