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Appl Environ Microbiol. 1968 May; 16(5): 689-690
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Modified Littman Oxgall Agar to Isolate Cryptococcus neoformans¹

Department of Pathology, Parasitology, and Public Health, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66502

ABSTRACT

Littman Oxgall Agar was modified by adding an extract of Guizotia abyssinica seeds with a water diluent for rehydration. In this medium, colonies of Cryptococcus neoformans became brown, but the color failed to develop in eight other yeasts and yeast phases of diphasic organisms. The depression of saprophytic fungi noted on Littman Oxgall Agar was not lost in the modification.

² Present address: Hq, Fourth U.S. Army, Office of the Surgeon, Attn: AKASU-V, Fort Sam Houston, Tex. 78234.

¹ Contribution No. 193, Department of Pathology, Parasitology, and Public Health, Kansas Agriculture Experimental Station, Manhattan, Kansas.