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Appl Environ Microbiol. 1968 August; 16(8): 1245-1250
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Production of Highly Labeled Adenoviruses

J. Paul Burnett, A. O. Summers1, J. A. Harrington and A. C. Dwyer

Lilly Research Laboratories, Indianapolis, Indiana 46206

ABSTRACT

A method is described for increasing the incorporation of radioactive thymidine into adenovirus deoxyribonucleic acid by the use of amethopterin. In addition, a modified procedure is presented for the preparation of highly purified adenoviruses. This procedure, which employs enzymatic digestion of cellular debris, obviates the necessity for fluorocarbon treatment of crude virus suspensions, and routinely provides excellent recovery of virus.

FOOTNOTES

1 Present address: Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Mo. 63103.

Appl Environ Microbiol. 1968 August; 16(8): 1245-1250
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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Copyright © 1968 by the American Society for Microbiology. All rights reserved.