This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by John, T. J. Articles by Fulginiti, V. A. Search for Related Content PubMed Articles by John, T. J. Articles by Fulginiti, V. A. Agricola Articles by John, T. J. Articles by Fulginiti, V. A.

Next Article 

Appl Environ Microbiol. 1968 September; 16(9): 1265-1268
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Enhancement of Parainfluenza 2 Virus Hemagglutinin

University of Colorado Medical Center, Department of Pediatrics, Denver, Colorado 80220

ABSTRACT

A comparison was made of the three previously described methods of enhancement of parainfluenza 2 virus hemagglutinin (HA): ultrasonic oscillation, adsorption to erythrocytes and elution (AE), and Tween-ether (TE) treatment. TE treatment resulted in the maximal elevation of HA titer, complete loss of infectivity, and the maximal loss of heat stability of HA. Ultrasonic oscillation caused the least elevation of HA titer, minimal loss of infectivity, and the least loss of heat stability of both HA and infectivity. The effect of AE remained intermediate. It is suggested that the changes produced by these three methods are varying degrees of disruption of virus particles. Growth studies on parainfluenza 2 virus are also presented.

¹ Present address: Enterovirus Laboratory, Christian Medical College and Hospital, Vellore, South India.