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Appl Environ Microbiol. 1969 January; 17(1): 41-47
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Vaccinia Neutralization Test Using a Radioisotope Assay for Virus-induced Enzyme Activity

National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

Vaccinia virus induction of a metabolic activity in host cell cultures forms the basis of a new assay for neutralizing antibodies. A direct relationship between the amount of vaccinia virus infecting cell cultures and the induced incorporation of tritium-labeled thymidine into the acid-insoluble fraction of the cells provided an indicator system. A liquid scintillation spectrometer was used to determine radioactivity associated with cell materials, and it provided a method for partial automation of an immunological procedure. Reproducibility of the method was satisfactory, and agreement with conventional vaccinia serum neutralization tests was demonstrated.