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Appl Environ Microbiol. 1969 February; 17(2): 193-196
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Patulin Production by Penicillium urticae Bainier in Batch Culture¹

Agricultural Research Service, U.S. Department of Agriculture, Grand Junction, Colorado 81501
U.S. Department of Agriculture, Lincoln, Nebraska 68503

ABSTRACT

A still, batch-culture method, with potato dextrose medium and Penicillium urticae Bainier, produced patulin yields of 1.2 to 1.7 g/liter of medium. Incubation was at 25 C for 14 days. Ethyl acetate extraction of condensed culture filtrate and drying with anhydrous MgSO₄, followed by solvent change to dry ethyl ether and purification on alumina (pH 4.5), produced pure crystalline patulin. The use of 2-liter, round-bottom flasks and a rotating vacuum evaporator provided versatile equipment and easy manipulation in the operations. Soil from wheat fields provided a convenient natural P. urticae source. Potato dextrose medium was superior to potato sucrose or Raulin-Thom media.

¹ Contribution from the Soil and Water Conservation Research Division, Agricultural Research Service, U.S. Department of Agriculture, in cooperation with the Nebraska Agricultural Experiment Station, Lincoln. Published as paper no. 2361, Journal Series, Nebraska Agricultural Experiment Station.