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Appl Environ Microbiol. 1969 February; 17(2): 232-236
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Separation of the Proteolytic and Streptolytic Components of a Streptomyces albus Enzyme Preparation and Their Effect on Streptococcal Cells

Department of Microbiology, The University of Texas Medical Branch, Galveston, Texas 77550
The Shriners Burns Institute, Galveston, Texas 77550

ABSTRACT

The complex of enzymes elaborated by a strain of Streptomyces albus was separated by Sephadex G-75 column chromatography. Two major and one minor proteolytic peaks and one streptolytic peak were obtained. The streptolytic fraction alone did not cause lysis of the cells, but was highly effective in the presence of small amounts of trypsin or of the proteolytic components of the enzyme complex. Treatment of cells with the purified streptolytic fraction effected release of group carbohydrate and of a trypsin and pepsin-resistant protein antigen which reacted with type-specific antisera.

¹ James W. McLaughlin Fellow in Infection and Immunity.