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Appl Environ Microbiol. 1969 February; 17(2): 246-249
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Arbovirus Plaquing in a Clonal Line (PS Y-15) of Porcine Kidney

¹ Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510

ABSTRACT

Plaque development of 42 arbovirus serotypes, representing 17 serogroups, was studied in a clonal line (PS Y-15) of porcine kidney. The arboviruses plaqued within 3 to 7 days, with plaque diameters ranging from 1 to 5 mm. Mouse mortality and cell infectivity end points were compared with 24 arboviruses. Arbovirus LD₅₀ end points in mice exceeded the porcine kidney monolayer plaque-forming unitage end point with all 24 viruses compared.