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Appl Environ Microbiol. 1969 February; 17(2): 250-251
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Intraperitoneal Injection of Mice

Department of Infectious Diseases, Human Research and Development Laboratories, Dow Chemical Company, Indianapolis, Indiana 46077

ABSTRACT

Steward, Ornellas, Beernink, and Northway (2) reported a 14% error in the placement of intraperitoneal injections of mice. They considered this error inherent in the technique and not simply correctable. We similarly found an error in the intraperitoneal injection of mice and attempted to identify the cause of error by varying such technical procedures as size of needle, site of penetration (through lower left versus lower right quadrant), investigator, angle of needle to the abdominal wall, and speed of injection. None of these technical modifications consistently eliminated or reduced the error of placement.