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Appl Environ Microbiol. 1969 February; 17(2): 256-261
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Survival of T3 Coliphage in Varied Extracellular Environments. I. Viability of the Coliphage During Storage and in Aerosols1

James C. Warren and Melvin T. Hatch

Naval Medical Research Unit # 1, Naval Supply Center, Oakland, California 94625
Naval Biological Laboratory, School of Public Health, University of California, Berkeley, California 94720

ABSTRACT

The objective of this study was to determine the feasibility of using airborne T3 coliphage as a viral tracer in microbial aerosols. Although T3 coliphage was relatively stable when stored either at temperatures ranging from 21 to 37 C or in the frozen state at -20 C, there was a 2-log loss in infectivity when stored for 72 days at 4 C. Either agitation of stored coliphage suspensions held at 31 C or wide fluctuations in storage temperature produced an increased loss of infectivity. In the airborne state, freshly prepared coliphage and stored coliphage behaved similarly, with survival diminishing as the relative humidity (RH) was lowered. The greatest loss occurred during the first five min following aerosolization. The results showed that only under certain conditions of temperature and relative humidity can T3 coliphage be used as a satisfactory aerosol tracer.

FOOTNOTES

1 A preliminary account of this work was presented at the 68th Annual Meeting of the American Society for Microbiology, 5-10 May 1968, Detroit, Mich.

Appl Environ Microbiol. 1969 February; 17(2): 256-261
Copyright © 1969 American Society for Microbiology. All Rights Reserved.





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