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Appl Environ Microbiol. 1969 February; 17(2): 275-279
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Antibody Response of Animals to Mycoplasma pneumoniae Measured by Latex Agglutination

^a Department of Biologics Research, Chas. Pfizer & Co., Inc., Terre Haute, Indiana 47808

ABSTRACT

The standardization, application, and usefulness of latex agglutination for Mycoplasma pneumoniae antibody titration were investigated and compared with tetrazolium reduction inhibition and complement fixation. The sera of guinea pigs and monkeys reacted in a specific fashion, whereas rabbit serum required pretreatment to eliminate its nonspecific agglutinin. Human serum given such pretreatment still contained a nonspecific agglutinin. The latex agglutination procedure compared favorably with complement fixation and metabolic inhibition tests for evaluating vaccine antigenicity.

¹ Present address: Cancer Chemotherapy Department, Microbiological Associates Inc., Bethesda, Md. 20014.