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Appl Environ Microbiol. 1969 March; 17(3): 384-393
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

Naval Biological Laboratory, School of Public Health, University of California, Berkeley, California 94625

ABSTRACT

The indirect fluorescent antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in 18 to 45 hr after inoculation of slide microtissue culture.