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Appl Environ Microbiol. 1969 April; 17(4): 524-527
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Cell Dispersal Methods for Increasing Yield from Animal Tissues

^a Biological Laboratories, Chas. Pfizer & Co., Inc., Terre Haute, Indiana 47808

ABSTRACT

An improved method for increasing cell yield from animal tissues is described. The current technique differs from the classical, primarily in that the tissues were perfused with trypsin in situ and cell dispersal was completed by trypsinization either at 4 or 37 C but without prior mincing of the tissue. Increased yields of 75% were obtained. Mechanical damage to tissue by mincing was presumed to be the greatest single source of injury to cells. The adoption of the trypsin perfusion technique reduced the number of animals needed to prepare an equivalent quantity of tissue culture.

¹ Present address: Metabolic Research Foundation Inc., 4520 Yoakum Blvd., Houston, Tex. 77006.