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Appl Environ Microbiol. 1969 April; 17(4): 556-558
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Standardized Procedure for Determination of Staphylocoagulase

Z. Wegrzynowicz, J. Jeljaszewicz and B. Lipinski

State Institute of Hygiene, Department of Bacteriology, Warsaw 36, Poland

ABSTRACT

A simple standardized procedure for the determination of staphylocoagulase, based on thrombin clotting time measurement with euglobulin, has been developed. A standard euglobulin solution containing 0.25% of clottable protein, 0.01 M {varepsilon}-aminocaproic acid, and 20 units of heparin per ml is employed. One unit of staphylocoagulase is defined as the amount of the enzyme which clots this standard euglobulin solution in 25 sec.

Appl Environ Microbiol. 1969 April; 17(4): 556-558
Copyright © 1969 American Society for Microbiology. All Rights Reserved.





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