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Appl Environ Microbiol. 1969 April; 17(4): 563-567
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Factors Affecting the Passive Hemagglutination Titration: Dilution Loops, Titration Trays, Vibration, Diluents¹

Department of Molecular Biology, Abbott Laboratories, North Chicago, Illinois 60064
Department of Microbiology, university of Kansas, Lawrence, Kansas 66045

ABSTRACT

Serial dilution with Takatsy loops resulted in exaggerated passive hemagglutination titers with most of the anti-bovine serum albumin sera tested. It appears that certain types of agglutinins adhere to the loop surface and are released only gradually. This adherence, or carry-over effect, was prevented by presoaking loops in gelatin or gelatin-rabbit serum-albumin solutions. Hemolysins did not adhere to loops. In general, hemagglutination reactions performed on plastic trays gave higher titers than those performed in glass test tubes. The quality of the hemagglutination pattern was dependent to a great extent on the type of plastic tray used. As much as a 100-fold difference in titers was obtained depending on the composition of the antiserum diluent. The increase in vibration, in terms of linear displacement (approximately twofold), resulted in an eightfold decrease in titers.

¹ This study was performed in part while one of the authors (L.R.D.) was associated with the National Cancer Institute, National Institutes of Health.