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Appl Environ Microbiol. 1969 April; 17(4): 611-613
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Microneutralization Test for Determination of Rhinovirus and Coxsackievirus A Antibody in Human Diploid Cells

¹ Ecological Investigations Program, National Communicable Disease Center, Kansas City, Kansas 66103

ABSTRACT

A method for determination of serum-neutralizing antibody titers to rhinovirus type 16 and coxsackievirus type A-4 in human diploid cells (WI-38) grown in Microtiter plates is described. A good correlation was observed when comparing neutralization tests in WI-38 and other cells. The WI-38 Microtiter method is relatively simple and economical. It is suitable for those laboratories which are required to conduct large-scale serological evaluation of antibodies for rhinoviruses and coxsackie A viruses. This system also can be used for cytomegalovirus and varicella-zoster virus, which grow relatively slowly.