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Appl Environ Microbiol. 1969 May; 17(5): 665-670
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Statistical Approach to Evaluating the Method of Morgan and Vryonis for Enumerating Treponema pallidum

Venereal Disease Research Laboratory, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

The contributions of four components to the error in the microscopic enumeration of spirochetes (Treponema pallidum) by the method of Morgan and Vryonis were determined. Where N_s = the number of slides counted, N_r = the number of recounts of 50 fields per slide, N_p = the number of pipettes used, and N_e = the total number of counts of 50 fields made, the coefficient of variation is 2 times the square root of (17.5²/N_s + 18.6²/N_r + 14.8²/N_p + 6.1²/N_e) with 95% confidence.