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Appl Environ Microbiol. 1969 May; 17(5): 726-733
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Automated Technique for the Detection of Hemagglutinins to Sheep Erythrocytes

¹ Department of Chemotherapy, Hoffmann-La Roche Inc., Nutley, New Jersey 07110

ABSTRACT

The Technicon autoanalyzer system used for the detection of homologous human hemagglutinins has been modified for the detection of hemagglutinins to sheep red blood cells. Antibody quantitation was obtained by plotting the optical density (OD) readings of peak heights of a serially diluted reference serum versus the serum dilutions. When the OD of the peak height of an unknown sample fell within the linear portion of the plot, a direct determination of the antibody titer was made. If the OD of the sample fell outside the linear portion, dilutions of the sample were carried out until a direct reading could be made. Assay by this method of at least 140 samples was possible within a day. Titers obtained with the autoanalyzer agreed very closely with those obtained by manual titration.