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Appl Environ Microbiol. 1969 June; 17(6): 790-796
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Procedure for the Evaluation of the Virucidal Effectiveness of an Ethylene Oxide Gas Sterilizer

Robert W. Sidwell, Glen J. Dixon, Louise Westbrook and Elizabeth A. Dulmadge

Virus and Cell Biology Divisions, Southern Research Institute, Birmingham, Alabama 35205

ABSTRACT

A quantitative, reproducible method was developed for the evaluation of the virucidal activity of test gases. Using this method, we determined the virucidal effectiveness of a Steri-Vac ethylene oxide gas sterilizer. Wool gabardine material was exposed to high concentrations of herpes simplex, vaccinia, parainfluenza, or polio viruses and was processed through the sterilizer. Two time-temperature cycles of the machine, 29 C for 180 min and 60 C for 48 min, were used in separate experiments. The viruses were exposed to the gas when freshly pipetted onto the fabric or when pipetted on the material and allowed to dry 16 to 24 hr. In two experiments carried out under each condition, the virus titers were reduced by the sterilization process to less than detectable limits. These titer reductions were for the herpes virus ≥ 2.7 to 5.0 log, for vaccinia virus ≥ 4.0 to 6.1 log, for parainfluenza virus ≥ 1.8 to 4.9 log, and for poliovirus ≥ 4.9 to 7.7 log. The observed reductions in virus titers were the same whether the virus-contaminated fabrics were sealed in polyethylene packages or held in open petri dishes during exposure to ethylene oxide.

Appl Environ Microbiol. 1969 June; 17(6): 790-796
Copyright © 1969 American Society for Microbiology. All Rights Reserved.





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Copyright © 1969 by the American Society for Microbiology. All rights reserved.