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Appl Environ Microbiol. 1969 June; 17(6): 857-860
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Combined Effects of Diphenyliodonium Chloride, Pine Oils, and Mustard Oil Soaps on Certain Microorganisms

Central Laboratories, Pakistan Council of Scientific and Industrial Research, Karachi 32, Pakistan

ABSTRACT

Bactericidal and bacteriostatic activities of an emulsion containing 10.0% (v/v) terpineol, 0.5% (w/v) diphenyliodonium chloride, 11.0% (v/v) ethyl alcohol, and 5.62% saponified mustard oil were tested against a number of different types of organisms. The bactericidal concentration for Salmonella typhosa was 1:400. In the presence of 5.0% horse serum, it increased to 1:250. The bacteriostatic concentration varied from organism to organism; Escherichia coli and Staphylococcus aureus required 4,000 µg/ml for complete bacteriostasis, whereas Corynebacterium diphtheriae, Salmonella paratyphi-A, and Shigella required only 2,000 µg/ml for complete inhibition. A 4.0% concentration of the emulsion killed the spores of Bacillus subtilis within 6 hr.