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Appl Environ Microbiol. 1969 June; 17(6): 878-880
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Effect of Incubation Temperature on T-Agglutination Typing of Streptococcus pyogenes

Streptococcal Bacteriology Unit, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

The temperature of incubation affected the typability of beta-hemolytic group A streptococci by T-agglutination tests. When strains could not be typed after routine incubation at 30 C, they were incubated at 22 to 25 C, and nearly a 10% increase in typability was achieved. The clinical source of the strains was related to their typability. Incubation at the lower temperature was required for successful typing of higher percentages of strains from the skin and other clinical sources than from the throat. Sixty per cent of the skin strains were represented by six serotypes. Of these, 53% of the strains required incubation at 22 to 25 C before they could be typed.