AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Satterlee, L. D.
Right arrow Articles by Walker, H. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Satterlee, L. D.
Right arrow Articles by Walker, H. W.
Agricola
Right arrow Articles by Satterlee, L. D.
Right arrow Articles by Walker, H. W.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1969 August; 18(2): 240-244
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Response of White Mice to Cells and Culture Constituents of Clostridium perfringens1

L. D. Satterlee2 and H. W. Walker

a Department of Food Technology, Iowa State University, Ames, Iowa 50010

ABSTRACT

Broth cultures of Clostridium perfringens (ATCC 10543) were fractionated by ammonium sulfate precipitation and Sephadex G-150 chromatography. Components isolated, as well as some enzymes present in the culture, were assayed for toxicity by feeding to white mice. Early work indicated that when a meat-fat-starch slurry, infected with C. perfringens, was fed to mice, the intestinal passage time was reduced. By using large numbers of mice as test animals and analyzing the data statistically, we found that C. perfringens and several fractions from the culture supernatant significantly affected the mice. A toxic material present in the supernatant was not identifiable as phospholipase C. Phospholipase C and physphorylcholine affected the intestinal passage time of the mice only when large amounts were given. The enzyme, neuraminidase, and another unidentified compound present in the supernatant affected the passage time when very small amounts were fed to mice.

FOOTNOTES

2 Present address: Department of Food Science and Technology, University of Nebraska, Lincoln, Nebraska 68503.

1 Journal paper no. I-6218 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, project 1393.

Appl Environ Microbiol. 1969 August; 18(2): 240-244
Copyright © 1969 American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1969 by the American Society for Microbiology. All rights reserved.