This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sharabi, N. E.-D. Articles by Bordeleau, L. M. Search for Related Content PubMed Articles by Sharabi, N. E.-D. Articles by Bordeleau, L. M. Agricola Articles by Sharabi, N. E.-D. Articles by Bordeleau, L. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1969 September; 18(3): 369-375
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Biochemical Decomposition of the Herbicide N-(3,4-Dichlorophenyl)-2-Methylpentanamide and Related Compounds

^a Department of Biochemistry and Microbiology, Rutgers, The State University, New Brunswick, New Jersey 08903

ABSTRACT

Organisms capable of decomposing N-(3,4-dichlorophenyl)-2-methylpentanamide (Karsil) were isolated, identified, and tested for their ability to hydrolyze this herbicide. Primary products of Karsil decomposition by cells and cell-free extracts of a Penicillium sp. were identified as 2-methyl-valeric acid and 3,4-dichloroaniline. The Karsil acylamidase (EC 3.5.1.a aryl acylamine amidohydrolase) was an induced enzyme. It was partially purified and tested for its ability to hydrolyze 25 related compounds. Some relations between the structures of these compounds and their susceptibility to enzymatic hydrolysis were discerned.

¹ Present address: University of Damascus, College of Agriculture, Damascus, Syria.