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Appl Environ Microbiol. 1969 September; 18(3): 433-437
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Preparation of La Crosse Virus Hemagglutinating Antigen in BHK-21 Suspension Cell Cultures

^a Arbovirus Unit, Virology Section, National Communicable Disease Center, Atlanta, Georgia 30333

ABSTRACT

Hemagglutinating and complement-fixing antigens of La Crosse virus (California arbovirus group) were produced in serum-free suspension cultures of BHK-21/13S cells. The appearance and production of these antigens were correlated with the titer of infectious virus. No significant differences in antigen titers were produced by varying virus dose 10-fold. Hemagglutinin appeared 6 to 8 hr after inoculation and reached peak titer in 14 to 22 hr. Both ß-propiolactone and Tween 80-ether treatment inactivated infectious virus in the antigens. Unlyophilized antigen was stable at -60, 5 and 24 C for at least 117 days but not for 1 year. Lyophilized antigen was stable for at least a year, however, at -20 and 5 C. Cell culture-produced antigen was more sensitive than brain-produced antigen in detecting hemagglutination inhibition antibody in human sera.

¹ On leave of absence from the Department of Virology, University of Turku, Turku, Finland.