This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Amici, A. M. Articles by Tognoli, L. Search for Related Content PubMed Articles by Amici, A. M. Articles by Tognoli, L. Agricola Articles by Amici, A. M. Articles by Tognoli, L.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1969 September; 18(3): 464-468
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Production of Peptide Ergot Alkaloids in Submerged Culture by Three Isolates of Claviceps purpurea

Istituto Richerche, Farmitalia, Milano, Italy

ABSTRACT

Three strains of Claviceps purpurea (Fr.) Tul., isolated from sclerotia grown on rye, produce under submerged conditions ergocryptine and ergotamine, ergocornine and ergosine, and ergocristine, respectively. All of the strains either lacked the ability to produce conidia or formed them sparingly, but they accumulated large quantities of lipids and sterols. The fermentations are typically divided into two phases. The first is characterized by the rapid utilization and exhaustion of the phosphate contained in the medium, rapid uptake of ammonium nitrogen and of citric acid, rapid growth, and low alkaloid production; the second phase is characterized by slower growth and by a marked accumulation of lipids, sterols, and alkaloids.