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Appl Environ Microbiol. 1969 September; 18(3): 496-499
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Complement-Fixing Antigen from BHK-21 Cell Cultures Infected with Lymphocytic Choriomeningitis Virus

^a Department of Microbiology, West Virginia University Medical Center, Morgantown, West Virginia 26506

ABSTRACT

Infection of BHK-21 cells with lymphocytic choriomeningitis (LCM) virus resulted in the production of significant titers of complement-fixing (CF) antigen. The antigen was spontaneously released from the cells, but the highest titer of 1:16 was recovered by disruption of the infected cells by freeze-thawing in tryptose phosphate broth. The antigen could be partially separated from infectious virus by centrifugation. Furthermore, it was possible to detect LCM virus infection of cell cultures by the production of the CF antigen, but this method proved less sensitive than titration by intracerebral inoculation of mice. The CF antigen from cell cultures was at least as sensitive and specific as the reference antigen prepared from infected guinea pig spleen.

¹ Present address: Department of Microbiology, Wayne State University, School of Medicine, Detroit, Mich. 48207.