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Appl Environ Microbiol. 1969 October; 18(4): 542-546
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Effect of Brilliant Green on the Motility of Salmonellae and Other Selected Microorganisms

Market Quality Research Division, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

ABSTRACT

The effect of Brilliant Green on motility was studied with Salmonella anatum, S. derby, S. tennessee, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa. Semisolid tryptic soy-agars containing 0, 20, or 40 mg of Brilliant Green per liter were used as the motility media. Both concentrations of Brilliant Green inhibited the growth of the non-Salmonella species through the semisolid agar. For 24 hr, the Brilliant Green appeared to limit the growth of the salmonellae; however, by 48 hr the salmonellae were able to grow through the semisolid agars. The presence of Brilliant Green in the motility media aided in the detection of Salmonella when mixed cultures were used.