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Appl Environ Microbiol. 1969 October; 18(4): 584-588
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Role of Interferon in the Propagation of MM Virus in L Cells

Infectious Diseases Branch, Biosciences Division, USAF School of Aerospace Medicine, Brooks Air Force Base, Texas 78235

ABSTRACT

MM virus propagated in mouse brain replicates to low titers in L cells without production of cytopathic effect (CPE). After growing the virus in BHK-21 cells, however, the virus replicates to high titers in L cells with complete CPE. It was found that suspensions of MM virus propagated in L cells directly from the mouse brain contained much more interferon than did suspensions of virus which had first been grown in BHK-21 cells. Mouse brain suspensions of the virus were also found to contain high interferon titers. Treatment of L cells with actinomycin D before infection with mouse brain-grown virus resulted in full virus replication with CPE. BHK-21 cell-grown virus diluted in L cell interferon behaved like mouse brain-grown virus in L cells. It is concluded that the presence of interferon in the inoculum is largely responsible for the suppression of MM virus replication in L cells.