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Appl Environ Microbiol. 1969 December; 18(6): 1036-1040
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Production and Properties of Galactosidases from Corticium rolfsii

Department of Agricultural Chemistry, Kagawa University, Kagawa-ken, Japan

ABSTRACT

When Corticum rolfsii was grown in a medium containing bran extract under aerobic conditions, it secreted -D-galactosidase and ß-D-galactosidase into the culture fluid. Pectin also stimulated the production of these enzymes, whereas galactose, glucose, and sucrose stimulated their production to a lesser degree. C. rolfsii produced greater amounts of both enzymes than Aspergillus niger. Both galactosidases in the culture medium hydrolyzed - and ß-p-nitrophenyl-D-galactosides as well as lactose, stachyose, melibiose, and raffinose. Both exhibited optimal activity at pH 2 to 4 and were quite stable under acidic conditions. -Galactosidase was separated from ß-galactosidase by column chromatography.