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Appl Environ Microbiol. 1969 December; 18(6): 1041-1043
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Production of Staphylococcal Enterotoxins A, B, and C in Various Media¹

^a Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The effect of initial pH and of length of incubation time at 37 C in four different growth media on the production of staphylococcal enterotoxins A, B, and C was determined. A starting pH of 6.8 gave higher yields of enterotoxins B and C than either pH 6.0 or 5.3. The production of enterotoxin A was, however, not materially affected by the low initial pH of 5.3. Prolonged incubation (48 to 72 hr) resulted only occasionally in higher yields of enterotoxin. The effect of the media on the amount of enterotoxin produced is considerable. Difco Brain Heart Infusion (BHI) was inferior to either Fisher BHI, 4% NZ Amine (NAK), or 3% NAK plus 3% protein hydrolysate powder at the three initial pH values, regardless of length of incubation time. The slight effect of the low starting pH on the production of enterotoxin A is being further investigated.

¹ Published with the approval of the Director of the Research Division, College of Agricultural and Life Sciences, The University of Wisconsin.