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Appl Environ Microbiol. 1969 December; 18(6): 991-993
Copyright © 1969 American Society for Microbiology. All Rights Reserved.
Health Services and Mental Health Administration, National Communicable Disease Center, Atlanta, Georgia 30333
ABSTRACT
Incorporation of methyl green dye into an agar medium containing deoxyribo-nucleic acid results in an improved medium for detecting deoxyribonuclease-producing bacteria. Use of the dye makes it unnecessary to use acid to demonstrate deoxyribonuclease activity, thus allowing subculture or reincubation of colonies. The improved medium is highly sensitive and can be used for primary isolation of Staphylococcus aureus or group A Streptococcus pyogenes.
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