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Appl Environ Microbiol. 1970 March; 19(3): 446-450
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Fluorimetric Technique for Monitoring Changes in the Level of Reduced Nicotinamide Nucleotides in Continuous Cultures of Microorganisms

Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

A metabolite fluorimeter was modified to monitor nicotinamide nucleotides in cells growing in a chemostat culture. By illuminating and collecting fluorescence over a relatively large area of the side of the culture vessel, the noise level was kept low even in highly turbulent cultures. Calibration of the system was by enzymatic assay of samples. Changes in nicotinamide nucleotide of concentration less than 0.1 nmoles/ml could be detected [i.e., less than 10% aerobic/anaerobic response for a cell concentration of 5 mg (dry wt) per ml].

¹ Present address: School of Chemical Engineering, University of Pennsylvania, Philadelphia, Pa.