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Appl Environ Microbiol. 1970 March; 19(3): 463-469
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

In Vitro and In Vivo Studies of Streptomycin-Dependent Cholera Vibrios

Delta Primate Research Center, Tulane University, Covington, Louisiana 70433

ABSTRACT

Streptomycin-dependent cholera vibrio strains were derived from Inaba, Ogawa, and NAG vibrios by the method of Mel. These phenotypes grew more slowly and attacked fermentable substances after a longer period of time than the streptomycin-sensitive parent strains. Rabbits injected with streptomycin-sensitive strains and their streptomycin-dependent forms showed homologous agglutinin production. Patas monkeys fed with 10⁹ streptomycin-dependent strains shed them for 1 to 2 days without ill effect, whereas the same number of streptomycin-independent organisms caused disease. The possibility of the application of multiple doses of streptomycin-dependent organisms in oral immunization against cholera was considered.

¹ Institute of Microbiology and Epidemiology, Prague, Czechoslovakia.

² Oregon State Board of Health, Portland, Ore.